Regulatory

Part:BBa_M50023:Design

Designed by: Elisa Liu   Group: Stanford BIOE44 - S11   (2016-10-27)


UV sensitive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 93


Design Notes

We wanted to use a promoter that is compatible with E. Coli. In addition, we have flanked the promoter with BSA1 sequences that will allow the BSA enzyme to cut the promoter into another plasmic.


Source

We are using the same promoter as the iGEM Columbian Team 2007.

References